Fact what we've done was all we had to do was just 60 an over culture we got dramatic alive into the micrographs Cesar focal images a Mike reversals growing and 3d scum arms the projection stack and so based on this we thought well if this is the case then the week control the with Mike reversals row by very the boundary conditions Black Label No in the culture and so the next I think we did was one that Bowl are referenced where we look at different boundary conditions so in this case we have an unconstrained Joe all the edges are free in this case we constrain the long axis and in this case we constrain all axe sand this case was the short and the long axis constrained in this was work done by my for Post Oak Lane Underwood and the interesting thing here is that when the long axis constrain is Joes are free to retract .

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